Development of a preclinical model of donation after circulatory determination of death for translational application

Table 1 Monitoring procedures during abdominal normothermic oxygenated recirculation

Monitored parameters	Objectives	Type of monitoring	Corrective actions
Pump flow rate	2.5 to 3 l/min	Continuous monitoring	Vascular filling: saline and hydroxyethyl starch (10 ml/(kg h))
Mean arterial pressure	>60 mmHg		Continuous infusion of noradrenalin on the circuit
Central venous pressure	<5 mmHg		—
pH	7.5	Blood samples on the circuit every 30 minutes	Continuous infusion of sodium bicarbonate on the circuit
Partial pressure of carbon dioxide	30 to 40 mmHg		Sweeping gas flow adjustment
Partial pressure of oxygen	100 to 150 mmHg		Fraction of inspired oxygen adjustment
Activated clotting time	>200 s		Bolus of 5000 IU unfractionated heparin on the circuit

ISSN: 2047-1440